HbA1c testing
These packs of 10 Roche cobas b 101 Test Discs measure HbA1c. They are incredibly efficient, offering a remarkably short testing time of just six minutes, utilising just a 2µl finger prick sample of blood.
Once the test has been completed, the results are displayed in two different formats: as a percentage of haemoglobin A1c according to the DCCT/NGSP guidelines, and as millimoles per mol of haemoglobin A1c according to the IFCC guidelines. This dual display of results makes it easy to interpret the data and obtain an accurate measurement of HbA1c levels.
Testing principle
The blood sample is diluted and mixed with TRIS buffer to release haemoglobin from the erythrocytes. A fraction of the sample is conveyed into a reaction chamber where it is mixed with sodium lauryl sulfate (SLS). SLS is used to form the SLS-hemoglobin complex. The concentration of total haemoglobin is calculated by measuring the SLS-haemoglobin complex with a wavelength of 525 nm. Hemoglobin A1c (HbA1c) in another fraction of the sample is first denaturated by potassium ferricyanide and sucrose laurate. The denatured HbA1c bonds with the HbA1c antibody on the latex particle. Latex agglutination inhibition reaction then occurs by reacting the agglutination that has synthetic antigen which can bond with HbA1c antibody. The concentration of HbA1c is calculated by measuring the latex agglutination inhibition reaction with a wavelength of 625 nm.
% haemoglobin A1c value is measured using a ratio of concentrations of HbA1c to total haemoglobin.
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